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Merck

L2380

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

lyophilized powder

Synonim(y):

Bandeirea simplicifolia agglutinin, BS-I

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Wybierz wielkość

1 MG

296,00 zł

5 MG

1030,00 zł

296,00 zł


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Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder

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form

lyophilized powder

potency

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

composition

Protein, ≥90% biuret

impurities

salt, free

storage temp.

2-8°C

Quality Level

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Ta pozycja
L3019L2895L5391
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

salt, free

impurities

-

impurities

-

impurities

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

potency

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

≤8 μg per mL agglutination activity (using human blood group B erythrocytes), ≥125 μg per mL agglutination activity (using human blood group A erythrocytes)

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

<200 μg per mL agglutination activity (using human blood group B erythrocytes)

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

composition

Protein, ≥90% biuret

composition

Protein, ~95% E1%/280

composition

Protein, ≥70% Lowry (balance sodium citrate)

composition

Protein, ~95% modified Warburg-Christian

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Preparation Note

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Karen Baker et al.
FEBS letters, 596(6), 762-771 (2022-02-01)
Calmodulin is a conserved calcium signalling protein that regulates a wide range of cellular functions. Amino-terminal acetylation is a ubiquitous post-translational modification that affects the majority of human proteins, to stabilise structure, as well as regulate function and proteolytic degradation.
Gheorghe Cojoc et al.
Scientific reports, 6, 25736-25736 (2016-05-12)
Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. The mechanisms that facilitate kinetochore capture by microtubules are still unclear. In the present study, we combine experiments
Daniela Regensburger et al.
Inflammatory bowel diseases, 27(9), 1491-1502 (2021-01-05)
The understanding of vascular plasticity is key to defining the role of blood vessels in physiologic and pathogenic processes. In the present study, the impact of the vascular quiescence marker SPARCL1 on angiogenesis, capillary morphogenesis, and vessel integrity was evaluated.
Sandra Moro et al.
Frontiers in microbiology, 12, 708354-708354 (2021-08-06)
Plasma membrane and membranous organelles contribute to the physiology of the Eukaryotic cell by participating in vesicle trafficking and the maintenance of ion homeostasis. Exomer is a protein complex that facilitates vesicle transport from the trans-Golgi network to the plasma
Holly R Brooker et al.
Journal of cell science, 131(15) (2018-06-23)
Life is dependent upon the ability of a cell to rapidly respond to changes in the environment. Small perturbations in local environments change the ability of molecules to interact and, hence, communicate. Hydrostatic pressure provides a rapid non-invasive, fully reversible

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