Unfortunately, the solubility and solution stability for this product is not determined. However, various sources state that the enzyme is stable when dissolved in human serum and frozen in aliquots. Please note this has not been validated internally.
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G8255
Glutamic-Pyruvic Transaminase from porcine heart
lyophilized powder, ≥75 units/mg protein
Synonim(y):
L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
200 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 566,00 zł |
1000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1750,00 zł |
2000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3010,00 zł |
Informacje o tej pozycji
Numer CAS:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-561-4
MDL number:
Beilstein/REAXYS Number:
3562140
Specific activity:
≥75 units/mg protein
Biological source:
Porcine heart
566,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
Porcine heart
Quality Segment
form
lyophilized powder
specific activity
≥75 units/mg protein
composition
Protein, 75-95%
technique(s)
cell culture | mammalian: suitable
color
light yellow to yellow
foreign activity
L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.1%
storage temp.
−20°C
General description
Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.
Research area: Cell Signaling
Research area: Cell Signaling
Application
Glutamic-Pyruvic Transaminase from porcine heart has been used:
- in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate[1]
- to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells[2]
- in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies[3]
Biochem/physiol Actions
Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.[4]
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.
Physical form
Lyophilized powder containing citrate and acetate
Preparation Note
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Analysis Note
Protein determined by biuret
Other Notes
One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source Porcine heart | biological source - | biological source Porcine heart | biological source - |
| specific activity ≥75 units/mg protein | specific activity 200-500 units/mg protein | specific activity ≥75 units/mg protein | specific activity 0.1-1.0 units/mg protein (Lowry) |
| form lyophilized powder | form ammonium sulfate suspension | form ammonium sulfate suspension | form buffered aqueous glycerol solution |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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