G7882

L-Glutamic Dehydrogenase from bovine liver

Type III, lyophilized powder, ≥20 units/mg protein

• Synonim(y): L-GLDH, L-Glutamate:NAD[P]⁺ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver
• Numer CAS: 9029-12-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-848-4
• MDL number: MFCD00131461
• Numer WE: 1.4.1.3 (BRENDA, IUBMB)
• Specific activity: ≥20 units/mg protein
• Biological source: bovine liver

Właściwości

• biological source: bovine liver
• Quality Segment: 200
• type: Type III
• form: lyophilized powder
• specific activity: ≥20 units/mg protein
• UniProt accession no.: P00366
• storage temp.: −20°C
• Gene Information: cow ... GLUD1(281785)

Opis

Application

L-Glutamic Dehydrogenase was used to catalyzes the conversion of isocitrate into a-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:

• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Packaging

Package size based on protein content

Physical form

Contains citrate and potassium phoshate buffer salts.

Analysis Note

Protein determined by biuret

Other Notes

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)