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G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, >= 550 units/mg protein (biuret)

Synonim(y):

G-6-P-DH

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Do Państwa/SKUDostępnośćCena netto
200 units
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302,00 zł
1000 units
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1150,00 zł
2000 units
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1680,00 zł
10000 units
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5800,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
>= 550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)

302,00 zł


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biological source

bacterial (Leuconostoc mesenteroides)

Quality Segment

type

Type XXIV

form

lyophilized powder

specific activity

>= 550 units/mg protein (biuret)

mol wt

128 kDa

composition

Protein, 15-40% biuret

application(s)

agriculture

foreign activity

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%

storage temp.

2-8°C

General description

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Biochem/physiol Actions

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
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Ta pozycja
G8529G5760G4134
description

lyophilized powder, >= 550 units/mg protein (biuret)

description

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

description

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

description

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

-

biological source

bacterial (Leuconostoc mesenteroides)

biological source

-

specific activity

>= 550 units/mg protein (biuret)

specific activity

≥550 units/mg protein (biuret)

specific activity

550-1,100 units/mg protein (biuret)

specific activity

200-400 units/mg protein (modified Warburg-Christian)

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

application(s)

agriculture

application(s)

agriculture

application(s)

-

application(s)

agriculture

mol wt

128 kDa

mol wt

-

mol wt

54 kDa

mol wt

128 kDa

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. What is the molecular weight of Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    1 answer
    1. The molecular weight of this enzyme has not been determined.  However, Biochem. Biophys. Res. Comm. 59, 894 (1974) reports a molecular weight of 55,000 daltons for the monomer.  The enzyme is functionally active as a dimer or tetramer.

      Helpful?

  2. In which solvent can Product No. G5885, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?

    1 answer
    1. The product can be dissolved at 1 mg/ml in 5 mM glycine buffer , pH 8, (with or without 0.1% BSA)or in deionized water at 1 mg/ml (buffer is preferable).

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can solutions of Product No. G5885, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc  mesenteroides, be stored?

    1 answer
    1. Solutions in buffer may be aliquotted and stored frozen at -20°C for about 1-2 months.

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  5. What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    1 answer
    1. Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

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