G1924

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli

• Synonim(y): L-Glutamate:oxygen oxidoreductase (deaminating)
• Numer CAS: 39346-34-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00131459
• Specific activity: ≥5 units/mg protein
• Recombinant: expressed in E. coli

Właściwości

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: solid
• specific activity: ≥5 units/mg protein
• mol wt: 120 kDa
• storage temp.: −20°C

Opis

General description

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

This product has a 6xHistidine tag with a thrombin cleavage site.

Application

L-Glutamate Oxidase from Streptomyces sp may be used:

• as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
• as a component of pencil graphite electrode for biosensing L-glutamate

Biochem/physiol Actions

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

Other Notes

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable