G0774
Glycerokinase from Bacillus stearothermophilus
buffered aqueous solution, ≥75 units/mg protein (biuret)
Synonim(y):
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-862-0
NACRES:
NA.54
MDL number:
Specific activity:
≥75 units/mg protein (biuret)
Assay:
≥5.0 mg protein/mL (biuret)
Biological source:
Bacillus sp. (Bacillus steaarothermophilus)
Pomoc techniczna
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Pozwól nam pomócbiological source
Bacillus sp. (Bacillus steaarothermophilus)
Quality Level
assay
≥5.0 mg protein/mL (biuret)
form
buffered aqueous solution
specific activity
≥75 units/mg protein (biuret)
storage condition
dry at room temperature
color
beige
application(s)
life science and biopharma
storage temp.
2-8°C
Gene Information
Bacillus sp. ... glpK(89613566)
General description
Research area: Cell Signaling
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Application
Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.
Biochem/physiol Actions
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.
Physical form
Stabilized solution in Tris buffer, pH 7.3
Other Notes
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Carol J Hartley et al.
PloS one, 12(11), e0184183-e0184183 (2017-11-08)
Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formation with the generation of two new stereo-centres, with
N Zwaig et al.
Science (New York, N.Y.), 153(3737), 755-757 (1966-08-12)
Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which
Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
N Zwaig et al.
Journal of bacteriology, 102(3), 753-759 (1970-06-01)
The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole
Anesthesia for a child suffering from a deletion in the Xp21 loci resulting in Duchenne disease, glycerol kinase deficiency, and congenital adrenal hypoplasia.
Luc J Van Obbergh et al.
Paediatric anaesthesia, 21(10), 1085-1087 (2011-10-11)
Powiązane treści
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| G0774-500UN | 04061832923970 |
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