G0251

β-Glucuronidase from bovine liver

Type B-1, ≥1,000,000 units/g solid

• Synonim(y): Bovine Liver beta-Glucuronidase, β-D-Glucuronide glucuronosohydrolase
• Numer CAS: 9001-45-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00130629
• Numer WE: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: ≥1,000,000 units/g solid

Właściwości

• type: Type B-1
• Quality Segment: 200
• form: solid
• specific activity: ≥1,000,000 units/g solid
• storage temp.: −20°C

Opis

General description

β-Glucuronidase (GUSB) is a lysosomal enzyme, found in animals, plants and bacteria. This exoglycosidase shows high activity in spleen, liver and kidney. Bovine liver β-glucuronidase (BLG) is homotetramer with a molecular weight of 290 kDa.

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

β-Glucuronidase from bovine liver has been used in β-glucuronidase inhibition assay to study the β-glucuronidase inhibitory effect of extracts and isolated actives from Phyllanthus amarus. It has also been used to perform deglucuronidation.

Biochem/physiol Actions

β-Glucuronidase (GUSB) deficiency can result in mucopolysaccharidosis type VII (MPSVII), which leads to store lysosome in the brain. Integration of β-glucuronides of steroid hormones into tissues can be done with the help of GUSB.

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)
• Kody zagrożeń: H334
• Ostrzeżenia: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Co się dzieje?: WGK 1