G8420

β-Glucuronidase from Escherichia coli

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Synonim(y): β-D-Glucuronide glucuronosohydrolase
• Numer CAS: 9001-45-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00199013
• Numer WE: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: ≥20,000 units/mg protein
• Recombinant: expressed in E. coli overproducing strain

Właściwości

• recombinant: expressed in E. coli overproducing strain
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥20,000 units/mg protein
• mol wt: 290 kDa
• composition: , ~25%
• storage temp.: −20°C

Opis

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)