This product is not considered lysine-fixable. The material would have to be chemically treated in order to be suitable. Typically, dextrans used to prepare lysine-fixable products are anionic (see product 90718). Should it be of interest, product B9139 is a Biotin-dextran, lysine-fixable product option. See the link below to review this product page:
https://www.sigmaaldrich.com/product/sigma/b9139
FD70S
Fluorescein isothiocyanate–dextran
average mol wt 60,000-76,000
Synonim(y):
FITC–Dextran
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100 MG
432,00 zł
250 MG
735,00 zł
1 G
2300,00 zł
5 G
8780,00 zł
432,00 zł
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Informacje o tej pozycji
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Pomoc techniczna
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Pozwól nam pomócbiological source
bacterial (Leuconostoc mesenteroides)
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 60,000-76,000
extent of labeling
0.003-0.020 mol FITC per mol glucose
color
yellow to yellow-orange
solubility
H2O: 25 mg/mL, clear to slightly hazy, yellow-orange
storage temp.
2-8°C
Quality Level
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Ta pozycja | |||
|---|---|---|---|
| biological source bacterial (Leuconostoc mesenteroides) | biological source synthetic | biological source synthetic | biological source bacterial (Leuconostoc mesenteroides) |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| solubility H2O: 25 mg/mL, clear to slightly hazy, yellow-orange | solubility - | solubility H2O: 25 mg/mL | solubility H2O: 25 mg/mL |
| form powder | form powder or crystals | form powder | form powder |
| storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| color yellow to yellow-orange | color - | color light yellow to dark orange | color light yellow to dark orange |
Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination.[1] Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability.[2] FITC-dextran 70 kDa (FD-70) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. FD-70 may be used in microvascular and cell monolayer permeability studies.[3]
Other Notes
Similar to FD-70, but produced by Sigma.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Chronic sleep restriction induces blood-brain barrier disruption and increases pro-inflammatory mediators in rodents. Those inflammatory mediators may modulate the blood-brain barrier and constitute a link between sleep loss and blood-brain barrier physiology. We propose that adenosine action on its A2A
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International journal of pharmaceutics, 555, 291-302 (2018-11-25)
Polyphosphates are important polyanionic electrolytes that play a major role in stabilization and consolidation of colloids surface and interior microstructures. In this study, the polyelectrolyte complexes (PEC) microcapsules (sodium cellulose sulfate-chitosan hydrochloride, sample 1), and the patched ones via sodium
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Vascular endothelial growth factor (VEGF) induces mild vasodilation and strong increases in microvascular permeability. Using intravital microscopy and digital integrated optical intensity image analysis, we tested, in the hamster cheek pouch microcirculation, the hypothesis that differential signaling pathways in arterioles
Powiązane treści
Dekstran jest polimerem anhydroglukozy. Składa się w około 95% z wiązań alfa-D-(166). Pozostałe (163) wiązania odpowiadają za rozgałęzienie dekstranu.
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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Is this dextran lysine-fixable?
1 answer-
Helpful?
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How long would a diluted stock (i.e. 5 mg/mL) be stable for at 4C?
1 answer-
The long term solution stability of this product has not been determined. Solutions should be protected from light. Per literature references, FITC-Dextran is stable in vivo for more than 24 hours. Please see the link below for additional information.
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-based-assays/fluorescein-isothiocyanate-dextranHelpful?
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