F7250

Fluorescein isothiocyanate isomer I

≥90% purity (HPLC), powder

• Synonim(y): FITC, Fluorescein 5-isothiocyanate
• Wzór empiryczny (zapis Hilla): C₂₁H₁₁NO₅S
• Numer CAS: 3326-32-7
• Masa cząsteczkowa: 389.38
• Beilstein: 1407295
• Numer WE: 222-042-0
• Numer MDL: MFCD00005063
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24894933
• NACRES: NA.47

Właściwości

• product name: Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
• Poziom jakości: 200
• Próba: ≥90% (HPLC)
• Postać: powder
• kolor: orange to dark orange
• mp: >360 °C (lit.)
• rozpuszczalność: acetone: 1 mg/mL
• fluorescencja: λ_ex 492 nm; λ_em 518 nm (green)
• przydatność: suitable for protein labeling
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: 2-8°C
• ciąg SMILES: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Klucz InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Opis

Opis ogólny

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Zastosowanie

Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves