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46950

Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Synonim(y):

FITC, Fluorescein isothiocyanate

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About This Item

Wzór empiryczny (zapis Hilla):
C21H11NO5S
Numer CAS:
27072-45-3
Masa cząsteczkowa:
389.38
Beilstein:
1407295
Numer WE:
248-207-7
Numer MDL:
MFCD00005063
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
329757273
NACRES:
NA.32

Poziom jakości

100

linia produktu

BioReagent

Próba

≥90% (HPLC)

fluorescencja

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

przydatność

suitable for fluorescence

temp. przechowywania

−20°C

ciąg SMILES

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

Klucz InChI

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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Opis ogólny

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Zastosowanie

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certyfikaty analizy (CoA)

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