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E1281

Sigma-Aldrich

Anti-Ezrin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-80K, Anti-Cytovillin, Anti-p81

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About This Item

Numer MDL:
MFCD00211933
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~80 kDa

reaktywność gatunkowa

human, rat

metody

immunohistochemistry: 20-40 μg/mL
immunoprecipitation (IP): 0.5-1 μg using RIPA extract of human A431 cells
indirect immunofluorescence: 10-20 μg/mL
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat NRK cells

numer dostępu UniProt

P15311

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)

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Opis ogólny

Ezrin is a 80kDa membrane-associated protein that belongs to ERM (Ezrin/Radixin/Moesin) family. Ezrin is found predominantly in polarized epithelial cells such as intestinal epithelial cells, gastric parietal cells, epithelial cells of the kidney proximal tubule, terminal bronchiole of the lung, and in mesothelia.

Immunogen

synthetic peptide corresponding to amino acids 479-498 of human ezrin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence in rat and mouse differs by three amino acids.

Zastosowanie

Anti-Ezrin antibody produced in rabbit has been used in:
  • immunolocalization studies
  • immunostaining
  • immunofluorescence and immunohistochemistry
  • immunoblot

Działania biochem./fizjol.

Cultured neuronal cells do express ezrin to some extent, and whole brain tissue expresses low levels of ezrin. Ezrin expression is associated with malignancy in astrocytoma, osteosarcoma, mammary and pancreatic adenocarcinomas, and in cutaneous and uveal melanomas.
Ezrin promotes cell adhesion. The NH2-terminal domain negatively regulates membrane spreading and elongation properties of ezrin COOH-terminal domain. The members of ERM family are usually involved in cross-linking of plasma membrane and actin filaments. ERM proteins are involved in cell adhesion, cell shape determination, migration, and the organization of cell surface structures. They are also involved in signal transduction and apoptosis. Ezrin has been implicated in tumor progression and metastatic spread.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A PKA-ezrin-Cx43 signaling complex controls gap junction communication and thereby trophoblast cell fusion
Pidoux G, et al.
Journal of Cell Science, 127(19), 4172-4185 (2014)
Jorge A Lobato-Álvarez et al.
Frontiers in physiology, 7, 450-450 (2016-10-25)
Na+, K+-ATPase, or the Na+ pump, is a key component in the maintenance of the epithelial phenotype. In most epithelia, the pump is located in the basolateral domain. Studies from our laboratory have shown that the β1 subunit of Na+
Suvi Ilmonen et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 18(4), 503-510 (2004-10-12)
Ezrin is a member of the ezrin-radixin-moesin family of proteins that link the actin-containing cytoskeleton to the plasma membrane. Ezrin is also connected to signaling molecules involved in the regulation of cell survival, proliferation and migration. Here, we examined the
M Martin et al.
The Journal of cell biology, 128(6), 1081-1093 (1995-03-01)
Overexpression in insect cells of the full coding sequence of the human membrane cytoskeletal linker ezrin (1-586) was compared with that of a NH2-terminal domain (ezrin 1-233) and that of a COOH-terminal domain (ezrin 310-586). Ezrin (1-586), as well as
A Bretscher
Current opinion in cell biology, 11(1), 109-116 (1999-02-27)
Molecules involved in ERM (ezrin-radixin-moesin) based attachment of membrane proteins to the cortical cytoskeleton in cell surface structures have been identified. In lymphocytes, a direct interaction is seen with extracellular matrix receptors and intercellular adhesion molecules. In polarized epithelial cells
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