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DNAQF

Sigma-Aldrich

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

Synonim(y):

dsDNA quantification

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About This Item

Kod UNSPSC:
41105500
NACRES:
NA.84

Poziom jakości

200

zastosowanie

sufficient for 750 assays (2 ml each)

metody

nucleic acid detection: suitable

temp. przechowywania

−20°C

Opis ogólny

The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.

Zastosowanie

DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
  • in neonatal rat ventricular myocytes (NRVMs)
  • in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
  • in mouse embryonic fibroblast cell line (NIH3T3 cells)

Cechy i korzyści

  • Specific for the quantitation of nanogram amounts of DNA
  • Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins
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Tylko elementy zestawu

Numer produktu
Opis
  • Detailed technical bulletin 1 ea

produkt powiązany

Numer produktu
Opis
Cennik

06693

Timestrip Plus™ -20 °C

Kod klasy składowania

10 - Combustible liquids

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