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D8168

Sigma-Aldrich

Monoclonal Anti-Dystrophin antibody produced in mouse

clone MANDYS8, ascites fluid

Synonim(y):

Dystrophin Antibody, Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse

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About This Item

Numer MDL:
MFCD00164586
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

200

białko sprzężone

unconjugated

forma przeciwciała

ascites fluid

rodzaj przeciwciała

primary antibodies

klon

MANDYS8, monoclonal

reaktywność gatunkowa

chicken, rat, human, pig, rabbit, mouse

metody

indirect ELISA: suitable
indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
microarray: suitable
western blot: suitable

izotyp

IgG2b

numer dostępu UniProt

P11532

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

Opis ogólny

Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.

Immunogen

recombinant human dystrophin fragment.

Zastosowanie

Monoclonal Anti-Dystrophin antibody produced in mouse has been used in
  • immunohistochemistry
  • immunofluorescence
  • double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
  • immunoblotting

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

Działania biochem./fizjol.

Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.

Opis wartości docelowych

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).

Inne uwagi

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

The dystrophin complex: structure, function, and implications for therapy
Gao QQ and McNally EM
Comprehensive Physiology, 5(3), 1223-1239 (2011)
Effects of long-term resveratrol-induced SIRT1 activation on insulin and apoptotic signalling in aged skeletal muscle
Sin TK, et al.
Acta Diabetologica, 52(6), 1063-1075 (2015)
Rachel Blitzblau et al.
Brain research, 1218, 21-34 (2008-06-06)
Muscular dystrophy patients often show cognitive impairment, in addition to muscle degeneration caused by dystrophin gene defects. The cognitive impairments lead to speculation that the dystrophin protein family may play a key role at neuronal synapses. Dystrophin regulates the stability
Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration
Kielbasa OM, et al.
Faseb Journal, 25(7), 2276-2286 (2011)
Viktoriia Kyrychenko et al.
JCI insight, 2(18) (2017-09-22)
Dystrophin maintains the integrity of striated muscles by linking the actin cytoskeleton with the cell membrane. Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene (DMD) that result in progressive, debilitating muscle weakness, cardiomyopathy, and a shortened
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