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D5648

Sigma-Aldrich

DMEM - high glucose

With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture

Synonim(y):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Postać

powder

metody

cell culture | mammalian: suitable

komponenty

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Zastosowanie

Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Ilość

Formulated to contain 13.4 grams of powder per liter of medium.

Rekonstytucja

Supplement with 3.7 g/L sodium bicarbonate.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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In-cell NMR can investigate protein conformational changes at atomic resolution, such as those changes induced by drug binding or chemical modifications, directly in living human cells, and therefore has great potential in the context of drug development as it can
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The complex yet interrelated connections between cancer metabolism, gene expression, and oncogenic driver genes have the potential to identify novel biomarkers and drug targets with prognostic and therapeutic value. Here we effectively integrated metabolomics and gene expression data from breast
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