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CSTZFND
CompoZr® Niestandardowa nukleaza z palcem cynkowym (ZFN)
ZFN plasmid only
Synonim(y):
CompoZr® Custom Zinc Finger Nuclease (ZFN)
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Informacje o tej pozycji
UNSPSC Code:
12352200
Note: This is a custom product. For more information on pricing and customization options, please fill out the form below and our Technical Sales Specialist will contact you directly.Request Information
product line
CompoZr®
shipped in
dry ice
storage temp.
−70°C
General description
Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell′s natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.
Application
Functional Genomics/Target Validation
- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
- Creation of cell lines that produce higher yields of proteins or antibodies
The CompoZr Custom ZFN service can be used to target genes from a variety of organisms. Validation of the custom ZFNs is completed in a proxy cell line for human, mouse, rat and hamster (CHO) projects. In this assay, the candidate ZFNs are delivered to the proxy cell line and activity is identified and measured. Activity is measured by amplifying over the ZFN target site and then using a nuclease mismatch enzyme to cut DNA strands that have been modified. ZFN efficiency can be measured based on the densitometry results of this assay. CompoZr Custom ZFN Projects for human, mouse, rat, and hamster (CHO) will have a production timeline of 10 weeks after the ZFN design is approved.
Other Notes
- Best Performing ZFN Pair
- ZFN Pair in Plasmid Form
- Forward and Reverse Primers that allow for determination of rate of mutation and for screening of individual clones
- Positive Control DNA
- Used to determine a baseline cutting efficiency
Legal Information
All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | ||
|---|---|---|
| description ZFN plasmid only | description glutamine synthetase | description dihydrofolate reductase |
| product line CompoZr® | product line CompoZr® | product line CompoZr® |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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