If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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Informacje o tej pozycji
UNSPSC Code:
41106514
Biological source:
human lung (lymphoblast)
Karyotype:
Not specified
Morphology:
Lymphoblast
Growth mode:
Suspension
Manufacturer/tradename:
ECACC
Informacje o cenach i dostępności nie są obecnie dostępne.
Pomoc techniczna
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Pozwól nam pomócbiological source
human lung (lymphoblast)
manufacturer/tradename
ECACC
packaging
tube of 5 μg 85011440-DNA-5UG, pkg of vial of cells 85011440-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Ludzka linia komórkowa U937 została wykorzystana do badania toksyczności ekstraktów propolisu przeciwko komórkom U937 oraz do badania wychwytu ciał apoptotycznych (pochodzących z monocytarnych komórek U-937) przez pierwotne komórki meningotelialne (PMEC).
Tumourigenicity studies
Biochem/physiol Actions
Dane STR-PCR: Amelogenina: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 14,15
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 14,15
Ludzki chłoniak histiocytarny rasy kaukaskiej
Pochodzi ze złośliwych komórek wysięku opłucnowego 37-letniego mężczyzny rasy kaukaskiej z rozlanym chłoniakiem histiocytarnym. Jedna z niewielu linii ludzkich nadal wykazujących wiele cech monocytarnych, charakterystycznych dla komórek pochodzenia histiocytarnego.
Preparation Note
Utrzymywać hodowlę w zakresie 2-9x100 000 komórek/ml; 5%CO2; 37°C. Konfluencja komórek może trwać do 72 godzin.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
W przypadku przesyłek w regionie Azji i Pacyfiku mogą obowiązywać dodatkowe opłaty za transport i obsługę. Więcej informacji można uzyskać u lokalnego przedstawiciela działu obsługi klienta.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011440, human lung (lymphoblast), Lymphoblast | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| morphology Lymphoblast | morphology Lymphoblast | morphology Lymphoblastoid | morphology Lymphoblastoid |
| biological source human lung (lymphoblast) | biological source mouse blood | biological source human blood | biological source human unknown/unspecified |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| growth mode Suspension | growth mode Suspension | growth mode Suspension | growth mode Suspension |
| storage temp. −196°C | storage temp. −196°C | storage temp. -140 to -196°C | storage temp. −196°C |
| receptors Not specified | receptors Not specified | receptors - | receptors Not specified |
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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Are my Cell Line cells mycoplasma tested?
1 answer-
ECACC routinely tests all manufactured cell banks for mycoplasma.
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