LIM1215

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 10092301, human colon, Epithelial

• Synonim(y): Linia komórkowa LIM1215
• UNSPSC Code: 41106514
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Właściwości

• biological source: human colon
• description: Colon carcinoma from human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 10092301-DNA-5UG, pkg of vial of cells 10092301-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Opis

Application

For complete product information, please see ECACC

Biochem/physiol Actions

Ludzki rak jelita grubego

Colorectal carcinoma from human colon

Preparation Note

Optymalny stosunek podziału 1:2-1:4 (gęstość wysiewu 2 x104 ^komórek/cm2). Komórki inkubowane w temperaturze 37°C z 5%_CO2. Zbierz komórki przy użyciu 0,05% trypsyny/EDTA w temperaturze 37°C przez 5 min.

RPMI1640 (z 2 mM L-glutaminą + 25 mM HEPES) + 10% FCS, insulina 0,6 g/ml, hydrokortyzon 1 μg/ml, 1-tioglicerol 10 μM

Other Notes

Depozytariusz i pomysłodawca: Tony Burgess - Ludwig Institute for Cancer Research, Australia

W przypadku przesyłek w regionie Azji i Pacyfiku mogą obowiązywać dodatkowe opłaty za transport i obsługę. Więcej informacji można uzyskać u lokalnego przedstawiciela działu obsługi klienta.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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