FTC-236

6030202, human thyroid (lymph node metastasis), Endothelial

• Kod UNSPSC: 41106514

Właściwości

• product name: FTC-236, 06030202
• pochodzenie biologiczne: human thyroid (lymph node metastasis)
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Endothelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Thyroid Cancer Neck Lymph Node Metastasis

Opis linii komórkowej

Derived from a neck lymph node metastasis of a follicular thyroid cancer (FTC).This cell line is tumourigenic in Balb/c mice. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

pożywka hodowlana

DMEM and Ham′s F12 (1:1 mixture) + 10ug/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Inne uwagi

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