C3138
Cathepsin D from bovine spleen
lyophilized powder, ≥2.0 units/mg protein
Synonim(y):
CTSD
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
bovine spleen
Próba
10—70% protein (biuret)
Postać
lyophilized powder
aktywność właściwa
≥2.0 units/mg protein
masa cząsteczkowa
~45 kDa
producent / nazwa handlowa
Sigma-Aldrich
metody
activity assay: suitable
kolor
dark brown
przydatność
suitable for molecular biology
numer dostępu UniProt
Zastosowanie
life science and biopharma
temp. przechowywania
−20°C
informacje o genach
cow ... CTSD(282883)
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Opis ogólny
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Zastosowanie
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
Działania biochem./fizjol.
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Definicja jednostki
One unit will produce an increase in A280 of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).
Postać fizyczna
Lyophilized powder containing citrate buffer salts
This page may contain text that has been machine translated.
inhibitor
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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