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Merck

C-12210

Sigma-Aldrich

Human Dermal Microvascular Endothelial Cells (HDMEC)

Juvenile foreskin, 500,000 cryopreserved cells

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About This Item

Kod UNSPSC:
41106514
NACRES:
NA.81

pochodzenie biologiczne

human foreskin (juvenile dermis)

opakowanie

pkg of 500,000 cells

morfologia

( endothelial)

metody

cell culture | mammalian: suitable

Warunki transportu

dry ice

temp. przechowywania

−196°C

Opis ogólny

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Pochodzenie linii komórkowej

Skin

Zastosowanie

Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of juvenile foreskin and are provided in a cryopreserved format. Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.

Jakość

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Ostrzeżenie

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutyna subkultury

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Inne uwagi

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Polecane produkty

Recommended Primary Cell Culture Media:Link

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Anil Kumar Mondru et al.
Cells, 12(6) (2023-03-30)
Extracellular-signal-regulated kinase 5 (ERK5) is critical for normal cardiovascular development. Previous studies have defined a canonical pathway for ERK5 activation, showing that ligand stimulation leads to MEK5 activation resulting in dual phosphorylation of ERK5 on Thr218/Tyr220 residues within the activation

Protokoły

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Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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