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Merck

A3059

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

Synonim(y):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.27

pochodzenie biologiczne

bovine

Próba

≥98%

Postać

lyophilized powder

masa cząsteczkowa

~66 kDa

oczyszczone przez

heat shock fractionation

opakowanie

poly bottle of

pochodzenie

USA origin

metody

ELISA: suitable
western blot: suitable

zanieczyszczenia

essentially globulin free
protease, none detected

strata

≤5%

pH

7

rozpuszczalność

water: soluble (40 mg/ml)

numer dostępu UniProt

obecność zanieczyszczeń

BT Virus, none detected
VSV Virus, none detected

temp. przechowywania

2-8°C

informacje o genach

bovine ... ALB(280717)

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Opis ogólny

Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
  • Protein quantitation (as a standard)
  • Immunochemistry (as a blocking agent)
  • Cell culture (as a nutrient)

This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques that include:
a) Enzyme immunoassay (EIA)
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)

Zastosowanie

Bovine Serum Albumin has been used in luminescence assays.

Działania biochem./fizjol.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Cechy i korzyści

  • Heat shock fractionated
  • Protease-free
  • Essentially globulin-free

Uwaga dotycząca przygotowania

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Leiting Pan et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(18), 1900865-1900865 (2019-09-29)
The dynamic response of the cell to osmotic changes is critical to its physiology and is widely exploited for cell manipulation. Here, using three-dimensional stochastic optical reconstruction microscopy (3D-STORM), a super-resolution technique, the hypotonic stress-induced ultrastructural changes of the cytoskeleton
Ming Shen et al.
Autophagy, 13(8), 1364-1385 (2017-06-10)
Oxidative stress-induced granulosa cell (GCs) death represents a common reason for follicular atresia. Follicle-stimulating hormone (FSH) has been shown to prevent GCs from oxidative injury, although the underlying mechanism remains to be elucidated. Here we first report that the suppression

Powiązane treści

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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