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A0521

α-Amylase from human saliva

greener alternative

Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

Synonim(y):

1,4-α-D-Glucan-glucanohydrolase

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Do Państwa/SKUDostępnośćCena netto
100 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
266,00 zł
500 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1520,00 zł
2500 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2910,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
Numer WE:
Specific activity:
1,000-3,000 units/mg protein

266,00 zł


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type

Type IX-A

Quality Segment

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

composition

Protein, 5.0-15.0% biuret

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

UniProt accession no.

greener alternative category

storage temp.

−20°C

Gene Information

human ... AMY1A(276)

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.

Application

α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).[1]

Biochem/physiol Actions

α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.

Physical form

Lyophilized powder containing (NH4)2SO4 and sodium citrate

Preparation Note

Chromatographically purified

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
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Ta pozycja
A1031A638010069
specific activity

1,000-3,000 units/mg protein

specific activity

300-1,500 units/mg protein

specific activity

≥1,500 units/mg protein (biuret)

specific activity

~380 U/mg

Gene Information

human ... AMY1A(276)

Gene Information

human ... AMY1A(276)

Gene Information

-

Gene Information

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

UniProt accession no.

P0DUB6

UniProt accession no.

P0DUB6

UniProt accession no.

-

UniProt accession no.

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. Good morning, I allow myself to contact you to know the mass of the product "α-amylase from Human Saliva" (A0521-100UN). It lacks the mass of the product and I would like to know it.

    1 answer
    1. The actual amount of powder per pack will depend on the units/mg protein of the lot received. This value will be provided in the lot-specific Certificate of Analysis. For example, lot SLCN0121 tested at 1474 units/mg protein. The 100UN pack size will provide 0.06mg of protein, the 500UN pack size will provide 0.34mg of protein, and the 2500UN pack size will provide 1.70mg of protein. Please see the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A0521-BULKSLCN0121.pdf

      Helpful?

  2. Dear Dr, how much mg saliva amylase are present in glass. We design the experimet sets. So need to know the Total amount of the amylase(mg) in the bottle. Thanks

    1 answer
    1. The exact amount is determined by the units/mg protein listed on the Certificate of Analysis. For example, the lot SLCN0121 tested at 1474 units per milligram protein. The total amount of the amylase (mg) in the bottle for the 100 unit package contains 0.07 mg, the 500 unit package contains 0.34 mg, and the 2500 unit package will contain 1.70 mg of protein. This lot is 11.8% protein. These values will change from lot to lot, but the actual mass in the bottles remains low. Other products, such as A4551, are sold by weight. See the link below to review this product page. https://www.sigmaaldrich.com/product/sigma/a4551

      Helpful?

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