A0410
Aminoallyl-dUTP sodium salt
≥85%, lyophilized powder
Synonim(y):
5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP
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About This Item
Wzór empiryczny (zapis Hilla):
C12H20N3O14P3 · xNa+
Numer CAS:
Masa cząsteczkowa:
523.22 (free acid basis)
Kod UNSPSC:
41106305
NACRES:
NA.51
Polecane produkty
Poziom jakości
Próba
≥85%
Postać
lyophilized powder
Warunki transportu
dry ice
temp. przechowywania
−70°C
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Zastosowanie
Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
- to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
- for the generation of chromatin immunoprecipitation (ChIP) probes
- in the reverse transcription of RNA for the synthesis of fluorescent labelled probes
Działania biochem./fizjol.
Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
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