4-Methylumbelliferyl N-acetyl-β-D-glucosaminide

Name: 4-Methylumbelliferyl N-acetyl-&#946;-<SC>D</SC>-glucosaminide
Brand: SIGMA
Price: 1480 PLN

fluorogenic, ≥99.0% (TLC), powder, suitable for fluorescence

Synonim(y):

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside

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250 MG

1480,00 zł

1 G

4950,00 zł

1480,00 zł

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):

C₁₈H₂₁NO₈

Numer CAS:

37067-30-4

Masa cząsteczkowa:

379.36

NACRES:

NA.32

PubChem Substance ID:

57652199

UNSPSC Code:

12352204

EC Number:

253-333-0

MDL number:

MFCD00150977

Beilstein/REAXYS Number:

1693397

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Nazwa produktu

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, suitable for fluorescence, ≥99.0% (TLC)

InChI

1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-,16-,17-,18-;;/m1../s1

InChI key

PAVCYMSNMRWMAK-DMYIEBNJSA-N

SMILES string

[H]O[H].[H]O[H].CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2

assay

≥99.0% (TLC)

form

powder

impurities

≤0.1% free 4-methylumbelliferone

solubility

DMF: 20 mg/mL, clear, colorless

fluorescence

λ_ex 317 nm (pH 10.0), λ_ex 365 nm; λ_em 445 nm in aqueous buffer pH 5.0 (after cleavage by β-N-acetylglucosaminidase)

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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Ta pozycja	M9659	M2133	M6018
Sigma-Aldrich 69585 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide	Sigma-Aldrich M9659 4-Methylumbelliferyl N-acetyl-β-D-galactosaminide	Sigma-Aldrich M2133 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide	Sigma-Aldrich M6018 4-Methylumbelliferyl β-D-cellobioside
assay ≥99.0% (TLC)	assay ≥98% (HPLC)	assay ≥98% (TLC)	assay ≥98.00% (TLC)
suitability suitable for fluorescence	suitability -	suitability -	suitability -
solubility DMF: 20 mg/mL, clear, colorless	solubility pyridine: 50 mg/mL, clear, colorless to faintly yellow	solubility DMF: 20 mg/mL, clear, colorless to faintly yellow	solubility water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

Application

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay.[1] It has also been used to measure the total activity of β-N-acetylhexosaminidase (NAGase) in water sample filtrates.[2]

Biochem/physiol Actions

4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic uncharged fluorogenic substrate for hexosaminidases.[3] It consists of chitin monomer (N-acetyl-β-D-glucosaminide) and 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF). 4-MUF-NAG is used for the detection and estimation of fungal growth by measuring the β-N-acetylhexosaminidase (NAGase) activity.[4]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Assay of β-N-acetylhexosaminidase.

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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Biochemical characterization of beta-hexosaminidase in different biological specimens from eleven patients with GM2-gangliosidosis B1 variant.

M G Ribeiro et al.

Journal of inherited metabolic disease, 14(5), 715-720 (1991-01-01)

GM2-gangliosidosis B1 variant is thought to be a rare disorder with a wide geographical and ethnic distribution. We report the biochemical findings obtained in different specimens from a group of 11 B1 variant patients originating from the north of Portugal.

A rapid method for detection of N-acetylglucosaminidase-type chitinase activity in crossed immunoelectrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels using 4-methylumbelliferyl-N-acetyl-D-glucosaminide as substrate.

K S Chen et al.

Electrophoresis, 15(5), 662-665 (1994-05-01)

A method for the detection of N-acetylglucosaminidase (GlcNAcase) activity has been developed by using 4-methyl-umbelliferyl-N-acetyl-D-glucosaminide (4-MU-GlcNAc) as substrate in crossed immunoelectrophoresis (CIE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Visualization of the reaction with a 366 nm ultra-violet light

Hexosaminidase assays.

Michaela Wendeler et al.

Glycoconjugate journal, 26(8), 945-952 (2008-05-14)

beta-Hexosaminidases (EC 3.2.1.52) are lysosomal enzymes that remove terminal beta-glycosidically bound N-acetylglucosamine and N-acetylgalactosamine residues from a number of glycoconjugates. Reliable assay systems are particularly important for the diagnosis of a family of lysosomal storage disorders, the GM2 gangliosidoses that

Tay-Sachs disease heterozygote detection: use of a centrifugal analyser for automation of hexosaminidase assays with two different artificial substrates.

E C Landels et al.

Journal of medical genetics, 28(2), 101-109 (1991-02-01)

An assay for measuring hexosaminidase A in serum and leucocytes is described in which a centrifugal analyser is used for automation of the enzyme assays after manual heat inactivation. The assay was used in a screening programme to identify heterozygotes

Purification and characterization of beta-N-acetylhexosaminidase I2 from human liver.

N N Dewji et al.

The Biochemical journal, 234(1), 157-162 (1986-02-15)

beta-N-Acetylhexosaminidase I2 was purified from human liver by a combination of concanavalin A chromatography, DEAE-cellulose chromatography, gel filtration and affinity chromatography on 2-acetamido-N-(6-aminohexanoyl)-2-deoxy-beta-D-glucopyranosylamine coupled to CNBr-activated Sepharose 4B. Its specific activity was 130 mumol/min per mg of protein compared with

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4-Methylumbelliferyl N-acetyl-β-D-glucosaminide