This enzyme catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.
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59747
Lactic Dehydrogenase, recombinant from E. coli
≥90 U/mg
Synonim(y):
(S)-Lactate: NAD+ oxidoreductase, L-Lactate Dehydrogenase, Lactate
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
25 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1400,00 zł |
100 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3800,00 zł 3230,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
Specific activity:
≥90 U/mg
Recombinant:
expressed in E. coli
1400,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócrecombinant
expressed in E. coli
Quality Segment
form
powder
specific activity
≥90 U/mg
storage temp.
−20°C
General description
LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.
Application
Lactic Dehydrogenase, recombinant from E. coli has been used:
- in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays[1]
- to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
- in in vitro DltC D-alanylation assay
Biochem/physiol Actions
L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.
Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
Other Notes
One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥90 U/mg | specific activity 1000 units/mL | specific activity ≥400 units/mg protein | specific activity ≥200 units/mg protein |
| form powder | form solution | form buffered aqueous glycerol solution | form ammonium sulfate suspension |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Does this enzyme catalyze the reaction of L-lactate to L-pyruvate, with NAD+ -> NADH as cofactors, or does it catalyze the conventional L-pyruvate to L-lactate with NADH -> NAD+?
1 answer-
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Can you provide more information on the biological source for recombinant LDH 59747? Does the certificate of origin specify whether the recombinant protein is from mice or another source? It seems that the certificate of origin does not offer detailed information to clarify this.
1 answer-
The material is Lactate dehydrogenase from chicken heart, expressed in recombinant microbes.
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I want to know the molecular weight of this protein
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This product is a recombinant enzyme from E. coli. The species and tissue of origin is the chicken heart. The molecular weight of this material has not been determined. However, according to Huston et al. 1972, Lactate Dehydrogenase from the bovine heart has a molecular weight of approximately 136,700 daltons.
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What species is Product No. 59747, Lactic Dehydrogenase, recombinant from E. coli?
1 answer-
This product is a recombinant Lactic Dehydrogenase from chicken heart made in E. coli.
Helpful?
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