PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• Synonim(y): PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• Kod UNSPSC: 12161700
• NACRES: NA.21

Właściwości

• product name: Phosphate Buffered Saline with 0.05% TWEEN^® 20, pH 7.4, pHast Pack^™, powder
• linia produktu: pHast Pack^™
• Postać: powder
• warunki przechowywania: (Tightly closed. Dry)
• zanieczyszczenia: ≤5 ppm Heavy Metals (lead)
• kolor: white
• pH: 7.2—7.6 (25 °C)
• rozpuszczalność: soluble, clear to hazy, colorless
• ślady kationów: Fe: <10 ppm
• obecność zanieczyszczeń: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temp. przechowywania: room temp

Opis

Opis ogólny

Phosphate Buffered Saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween^® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Zastosowanie

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.

Cechy i korzyści

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Opakowanie

Foil pouches

Rekonstytucja

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Przechowywanie i stabilność

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.

Informacje prawne

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable