76704

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect^® Plus, novobiocin 0.02 g/L, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

• Synonim(y): Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth, mTSB Broth with Novobiocin
• Kod UNSPSC: 41171607
• NACRES: NA.85

Właściwości

• agency: according to ISO 16654:2001
• Poziom jakości: 200
• sterylność: non-sterile
• Postać: powder
• okres trwałości: limited, expiry date on the label
• skład: bile salts No. 3, 1.5 g/L ; di-potassium hydrogen phosphate, 4.0 g/L ; D(+)-glucose, 2.5 g/L ; novobiocin, 0.02 g/L ; peptone from casein, 17.0 g/L ; peptone from soymeal, 3.0 g/L ; sodium chloride, 5.0 g/L
• producent / nazwa handlowa: NutriSelect^® Plus
• opakowanie: pkg of -500 g
• warunki przechowywania: (Dry place, tightly sealed containers)
• metody: microbiological culture: suitable
• kolor: faint brown
• pH końcowe: 7.3±0.2 (25 °C)
• Zastosowanie: agriculture; food and beverages; microbiology; pathogen testing
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• przydatność: selective for Escherichia coli; selective for coliforms; selective for enterovirulent E. coli (EEC)

Opis

Opis ogólny

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Zastosowanie

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Uwaga dotycząca przygotowania

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Przypis

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informacje prawne

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)