MABN72
Anti-pan-MAGUK Antibody, clone K28/86
clone K28/86, from mouse
Synonim(y):
discs, large homolog 4 (Drosophila), discs large homolog 4, disks large homolog 4, Tax interaction protein 15, post-synaptic density protein 95, Postsynaptic density protein 95, Synapse-associated protein 90
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
K28/86, monoclonal
reaktywność gatunkowa
rat
reaktywność gatunkowa (przewidywana na podstawie homologii)
human (immunogen homology), mouse (based on 100% sequence homology)
metody
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1λ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... DLG4(1742)
Opis ogólny
The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins.
Specyficzność
This antibody exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility, http://neuromab.ucdavis.edu)
Immunogen
Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.
Zastosowanie
Anti-pan-MAGUK Antibody, clone K28/86 is an antibody against pan-MAGUK for use in IH, WB & IP.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues.
Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)
Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Jakość
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.
Opis wartości docelowych
~ 85-110 kDa observed
Postać fizyczna
Format: Purified
Protein G
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
Rat brain tissue lysate
Rat brain tissue lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Mohammed M Nooh et al.
PloS one, 8(5), e63379-e63379 (2013-05-23)
Previous studies have determined that the type-1 PDZ sequence at the extreme carboxy-terminus of the ß1-adrenergic receptor (ß1-AR) binds SAP97 and AKAP79 to organize a scaffold involved in trafficking of the ß1-AR. In this study we focused on characterizing the
Hiu-Tung C Wong et al.
eLife, 8 (2019-10-15)
Sensory hair cells in the ear utilize specialized ribbon synapses. These synapses are defined by electron-dense presynaptic structures called ribbons, composed primarily of the structural protein Ribeye. Previous work has shown that voltage-gated influx of Ca2+ through CaV1.3 channels is
Agnik Dasgupta et al.
eLife, 12 (2024-08-12)
In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. In the lateral line sensory system of the zebrafish, developing sensory axons reorganize their terminal arborization patterns to establish precise neural
Iwona Ksiazek et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(27), 7183-7195 (2007-07-06)
Agrin-deficient mice die at birth because of aberrant development of the neuromuscular junctions. Here, we examined the role of agrin at brain synapses. We show that agrin is associated with excitatory but not inhibitory synapses in the cerebral cortex. Most
Kalyan C Tirupula et al.
PloS one, 10(10), e0140872-e0140872 (2015-10-21)
Propagation of signals from G protein-coupled receptors (GPCRs) in cells is primarily mediated by protein-protein interactions. MAS is a GPCR that was initially discovered as an oncogene and is now known to play an important role in cardiovascular physiology. Current
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