MAB10764

Anti-SBP-tag Antibody, clone 20 | MAB10764

clone 20, 1.0 mg/mL, from mouse

• Synonim(y): streptavidin binding peptide
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 20, monoclonal
• Species reactivity: human
• Application: ICC, WB
• Citations: 20

Właściwości

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 20, monoclonal
• species reactivity: human
• concentration: 1.0 mg/mL
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• shipped in: wet ice
• target post-translational modification: unmodified

Opis

General description

Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.

The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa

Immunogen

Epitope: Unknown

Linear peptide corresponding to SBP tag.

Application

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Biochem/physiol Actions

Demonstrated to react with cells expressing the SBP tag.

This antibody recognizes SBP tag.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
pTrAP-GFP transfected Hek293 lysate

Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.

Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable