Przejdź do zawartości
Merck

Kluczowe dokumenty

Wyświetl całą dokumentację

Przejdź do

EZHS100B

Human S100B ELISA Kit

quantifies S100B in 50 μL cerebrospinal fluid, serum or plasma

Synonim(y):

Protein S100B, S-100 protein beta chain, S100 calcium-binding protein B

Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

Zmień widok
Do Państwa/SKUDostępnośćCena netto
96 wells

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

4450,00 zł

Informacje o tej pozycji

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Input:
sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice

4450,00 zł

Dostępne do wysyłki DZISIAJSzczegóły

​
Złóż zamówienie na produkt na zamówienie
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc

Nazwa produktu

Human S100B ELISA, EZHS100B-33K, This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum & plasma.

Quality Segment

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

P04271

application(s)

research use

assay range

accuracy: 93%
(CSF), accuracy: 94%
(serum), sensitivity: 2.7 pg/mL, standard curve range: 2.7-2000 pg/mL, inter-assay cv: <4%
intra-assay cv: <4%

detection method

colorimetric (450nm)

shipped in

wet ice

storage temp.

2-8°C

General description

EMD Millipore’s Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum and plasma. This kit has not been tested for cell culture supernatants.
S100B is a calcium-binding protein produced mainly by astrocytes in the central nervous system and has been implicated in the development and maintenance of the nervous system. Although the mechanism of S100B secretion is unknown, it appears to be affected by oxidative stress. At nanomolar concentrations (normal levels), S100B is able to protect neurons against glutamate toxicity. Glial damage or astrocytic reactions to neural injury (reactive astrogliosis) causes an increase in S100B to micromolar concentrations. High levels of S100B will further increase neuronal damage. High levels of S100B are considered a biomarker that could indicate damage or dysfunction of CNS.

Application

Research Category
Neuroscience
This is a same day assay
Used to detect/quantify: S100B

Biochem/physiol Actions

The antibody pair used in this assay measures Human S100B and has no cross-reactivity with S100A1, S100A6 and S100A13.

This assay detects S100B in multiple species, including: bovine, hamster, horse, monkey, mouse, pig, rabbit, and rat.

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 1

Ta pozycja
ABN59S2532SAB5700647
Human S100B ELISA, EZHS100B-33K This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum &amp; plasma.

Millipore

EZHS100B

Human S100B ELISA, EZHS100B-33K

Przeciwciało anty-S100B from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN59

Przeciwciało anty-S100B

Monoclonal Anti-S-100 (&#946;-Subunit) antibody produced in mouse clone SH-B1, ascites fluid

Sigma-Aldrich

S2532

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse

Przeciwciało anty-S100Bprodukowane u królików

Sigma-Aldrich

SAB5700647

Przeciwciało anty-S100Bprodukowane u królików

species reactivity

human

species reactivity

human

species reactivity

feline, pig, bovine, sheep, goat, rabbit, canine, human, rat

species reactivity

rat, mouse, human

assay range

accuracy: 93%
(CSF), sensitivity: 2.7 pg/mL, inter-assay cv: <4%
intra-assay cv: <4%, accuracy: 94%
(serum), standard curve range: 2.7-2000 pg/mL

assay range

-

assay range

-

assay range

-

input

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

input

-

input

-

input

-

Quality Level

200

Quality Level

100, 300

Quality Level

200

Quality Level

100

technique(s)

ELISA: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

dot blot: suitable using denatured-reduced preparations, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using protease-digested sections of human tongue, indirect ELISA: suitable, microarray: suitable, radioimmunoassay: suitable

technique(s)

immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

detection method

colorimetric (450nm)

detection method

-

detection method

-

detection method

-

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Klasa składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Questions

1–2 of 2 Questions  

  1. Is the EZHS100B-33K ELISA assay compatible with human saliva samples?

    1 answer

    1. The kits have not been validated for use with saliva; however, some guidelines are available for processing saliva samples:


      1. Add protease inhibitor cocktail (Product P2714) at a 1:500 ratio to the saliva.
      2. Centrifuge the mixture at 10,000 rpm for 10 minutes.
      3. Dilute the supernatant in a 1:2 ratio with Assay Buffer before to setting up the assay.
      4. Run assay with Assay Buffer as the matrix for the standard curve.
      5. Multiply by 2 for the final result.


      It should be noted that there are no references available for the S100B ELISA kit being used with saliva samples.

      Helpful?

  2. Which antibodies have been used for the Human S100B ELISA kit (EZHS100B-33K)?

    1 answer

    1. The origins of the antibodies utilized in the kit are considered proprietary. Nevertheless, some information about the antibodies can be shared. The capture antibody is a monoclonal mouse anti-S100B, with bovine brain S100B used as the immunogen. The detection antibody is a polyclonal rabbit anti-S100, and the immunogen for this is also sourced from bovine brain S100.

      Helpful?

Reviews

No rating value

Active Filters