ECM675
3D Collagen Culture Kit
3D cell culture kit includes: Human Collagen, Mediums (RPMI, M199 and DMEM), PBS with Phenol Red and Neutralization Solution
Synonim(y):
3D Cell Culture Kit, Collagen Culture Kit, Kit for 3D Collagen Culture
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 kit | Przewidywany termin wysyłki22 maja 2026zKuehne + Nagel Sp. z o.o. | 2200,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32161000
Biological source:
rat
Form:
liquid
Technique(s):
activity assay: suitable, cell based assay: suitable, cell culture | mammalian: suitable
Pomoc techniczna
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Pozwól nam pomócbiological source
rat
Quality Level
form
liquid
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable, cell based assay: suitable, cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Cell migration is a fundamental function of normal cellular processes, including cell proliferation, and migration. Cell behavior in vitro is typically examined in a 2D environment; however, in the body, cells exist in a three-dimensional extracellular matrix environment rich in type I collagen. In fact, cells cultured in 3D matrices better reflect in vivo cell physiology when compared to traditional 2D systems.
CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
For Research Use Only; Not for use in diagnostic procedures
CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
For Research Use Only; Not for use in diagnostic procedures
Application
Research Category
Cell Structure
Cell Structure
This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
Physical form
Collagen solution is supplied in 6mM acetate buffer at ~ pH7.0.
Preparation Note
Store kit materials at 4°C up to their expiration date.
Other Notes
Collagen Solution - (Part No. 90135) One bottle - 10 mL
5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL
5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL
5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL
5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL
Neutralization Solution- (Part No. 90138) One vial - 0.5 mL
5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL
5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL
5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL
5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL
Neutralization Solution- (Part No. 90138) One vial - 0.5 mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) activity assay: suitable, cell culture | mammalian: suitable, cell based assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell based assay: suitable |
| biological source rat | biological source human cell culture | biological source Porcine | biological source - |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level 100 |
| form liquid | form solution | form - | form - |
| shipped in wet ice | shipped in dry ice | shipped in ambient | shipped in - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A
Klasa składowania
3 - Flammable liquids
wgk
WGK 2
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| ECM675 | 04053252516139 |