AB755P

Anti-Collagen Type I (COL1A1) Antibody

CHEMICON^®, rabbit polyclonal

• Synonim(y): Anti-CAFYD, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat
• Application: ELISA, ICC, IHC, RIA
• Citations: 48

Właściwości

• Nazwa produktu: Anti-Rat Collagen Type I Antibody, Chemicon^®, from rabbit
• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), radioimmunoassay: suitable
• isotype: IgG
• suitability: not suitable for Western blot
• NCBI accession no.: NM_000088.3
• UniProt accession no.: P02452
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... COL1A1(1277)

Opis

General description

Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Immunogen

Collagen Type I extracted and purified from rat skin.

Application

Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

RIA:
A previous lot of this antibody was used in RIA.

Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.

Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.

Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Rat Collagen Type I

The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Physical form

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.

Analysis Note

Control
Rat hepatic stellate cell lysate

Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.

IHC-Paraffin Staining With Epitope Retrieval: Rat Skin

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 13 - Non Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable