AB1543
Anti-Synapsin I Antibody
serum, Chemicon®
Synonim(y):
Synapsin-1
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
human, bovine, rat, mouse
producent / nazwa handlowa
Chemicon®
metody
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SYN1(6853)
Opis ogólny
Synapsin I is a member of the synapsin family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations of the Synapsin I gene may be associated with X linked disorders with primary neuronal degeneration such as Rett syndrome.
Specyficzność
Synapsin I (Mr of 80,000 and 77,000). Immunolabeling is blocked by preadsorption of antibody with Synapsin I. Synapsin is located only in brain nerve terminal and is an excellent marker for synapses.
Immunogen
Synapsin I (mixture of Ia & Ib) purified from bovine brain.
Zastosowanie
Detect Synapsin I using this Anti-Synapsin I Antibody validated for use in IC, IH(P), IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Western blotting:
1:200 of a previous lot was used for labeled Protein A, 1:1,000 dilution for ECL.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
5 μL of a previous lot quantitatively immunoprecipitated all synapsin in 200 μg of rat brain.
Optimal working dilutions must be determined by the end user.
1:200 of a previous lot was used for labeled Protein A, 1:1,000 dilution for ECL.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
5 μL of a previous lot quantitatively immunoprecipitated all synapsin in 200 μg of rat brain.
Optimal working dilutions must be determined by the end user.
Jakość
Immunohistochemistry(paraffin): Synapsin representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. A previous lot of this antibody was diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus
IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus
Opis wartości docelowych
77 & 80 kDa
Postać fizyczna
Rabbit polyclonal antiserum in buffer containing no preservatives.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
Mouse hippocampal primary neurons, Mouse brain lysates, PC-12 cells.
Mouse hippocampal primary neurons, Mouse brain lysates, PC-12 cells.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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