407241

Insulin Degrading Enzyme, His•Tag^®, Rat, Recombinant, S. frugiperda

• Synonim(y): Insulysin, IDE
• Numer WE: 3.4.24.56 (BRENDA, IUBMB)
• NACRES: NA.25
• UNSPSC Code: 12352202
• Specific activity: ≥3 units/mg protein
• Assay: ≥90% (SDS-PAGE)

Właściwości

• assay: ≥90% (SDS-PAGE)
• Quality Segment: 100
• form: liquid
• specific activity: ≥3 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: −70°C

Opis

General description

Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. Insulin degrading enzyme (Insulysin) is a metalloprotease that degrades insulin and a variety of other peptides, including glucagon, insulin-like growth factors I and II, atrial natriuretic peptide, TGF-α, and amyloid peptides. Reported to be inversely correlated with brain Aβ peptide levels in insulysin-deficient mice compared to wild-type mice.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Hersh, L.B. 2003. Curr. Pharm. Des.9, 449.
Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.
Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.
Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer′s Dis.4, 341.
Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.
Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.

One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1