322327

Diphtheria Toxin, CRM Mutant

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

• Synonim(y): Diphtheria Toxin, CRM Mutant
• Numer CAS: 92092-36-9
• UNSPSC Code: 12352202
• MDL number: MFCD00003009
• Form: solid
• Storage condition: OK to freeze, avoid repeated freeze/thaw cycles

Właściwości

• Quality Segment: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

CRM₁₉₇ is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM₁₉₇ protein is identical to the wild-type toxin.

Cross-reactive material of diphtheria toxin (CRM₁₉₇). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM₁₉₇ is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Mutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor

Reversible: no

Physical form

Lyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.

Please refer to vial label for lot-specific reconstitution volume.

Analysis Note

One major band by SDS-PAGE

Other Notes

Kusano, I., et al. 2001. Cell Struct. Funct.26, 279.
Kaefer, M., et al. 2000. J. Urol.163, 580.
Gupta, R.K., et al. 1997. Vaccine15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol.251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res.12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable