This product has not been tested for suitability as a culture medium component. However, heavy isotope-labeled amino acids are commonly utilized in SILAC medium. In standard formulations of Lysine Medium for microbiological culture, a final concentration of 1.0 g/L is typical. L-Lysine is soluble in water at 100 mg/mL. Similar rates would be expected when added directly to medium, however this has not been validated. Please see the link below to review a publication utilizing this product as a component in culture medium:
https://pmc.ncbi.nlm.nih.gov/articles/PMC7737663/
Przejdź do
608041
L-Lysine-13C6,15N2 hydrochloride
99 atom % 13C, 99 atom % 15N, 95% (CP)
Synonim(y):
SILAC Amino Acid, (S)-2,6-Diaminohexanoic acid-13C6,15N2 hydrochloride, 13C and 15N Labeled Lys, 13C and 15N Labeled lysine hydrochloride
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Informacje o tej pozycji
Wzór liniowy:
H215N(13CH2)413CH(15NH2)13CO2H · HCl
Numer CAS:
Masa cząsteczkowa:
190.59
NACRES:
NA.12
PubChem Substance ID:
UNSPSC Code:
12352209
MDL number:
Isotopic purity:
99 atom % 13C, 99 atom % 15N
Assay:
95% (CP)
Mass shift:
M+8
Form:
solid
Note: This product can be packaged on demand. For information on pricing, availability and packaging of custom sizes, please contact Stable Isotopes Customer Service
Quality Segment
isotopic purity
99 atom % 13C, 99 atom % 15N
assay
95% (CP)
form
solid
optical activity
[α]25/D +20.7°, c = 2 in 5 M HCl
technique(s)
bio NMR: suitable, protein expression: suitable
mp
263-264 °C (dec.) (lit.)
mass shift
M+8
storage temp.
room temp
SMILES string
Cl.[15NH2][13CH2][13CH2][13CH2][13CH2][13C@H]([15NH2])[13C](O)=O
InChI
1S/C6H14N2O2.ClH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;
InChI key
BVHLGVCQOALMSV-GSMNGUNQSA-N
General description
Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.
Application
- L-Lysine-13C6,15N2 hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
- It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
- It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).
Pulsed Stable Isotope labeling of amino acid has been used to study host-cell function, survival, the precise intracellular pathways in protein synthesis of HIV-1 infected human monocytederived macrophages.
Packaging
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact
Stable Isotopes Customer Service.
Stable Isotopes Customer Service.
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Ta pozycja | |||
|---|---|---|---|
| description 99 atom % 13C, 99 atom % 15N, 95% (CP) | description 98 atom % 15N, 98% (CP) | description 99 atom % 13C, 95% (CP) | description 98 atom % 13C, 98 atom % 15N, 95% (CP) |
| mass shift M+8 | mass shift M+2 | mass shift M+6 | mass shift M+7 |
| technique(s) bio NMR: suitable, protein expression: suitable | technique(s) protein expression: suitable, bio NMR: suitable | technique(s) protein expression: suitable | technique(s) protein expression: suitable, bio NMR: suitable |
| assay 95% (CP) | assay 98% (CP) | assay 95% (CP) | assay 95% (CP) |
| isotopic purity 99 atom % 13C, 99 atom % 15N | isotopic purity 98 atom % 15N | isotopic purity 99 atom % 13C | isotopic purity 98 atom % 13C, 98 atom % 15N |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solid | form solid | form solid | form solid |
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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1. Can we use this product for the preparation of bacteriological media? 2. The quantity of (volume) needs to be added/ 1000 ml?
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