This product is soluble in water at a concentration of 10 mg/mL. It can also be dissolved in a buffer according to specific experimental references, such as 50 mM potassium phosphate. The stability of the solution has not been tested.
X2252
Xanthine Oxidase microbial
lyophilized powder, ≥7 units/mg solid
Synonym(s):
Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
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| Size/SKU | Availability | Price |
|---|---|---|
25 units | Please contact Customer Service for Availability | PLN 658.00 |
100 units | Please contact Customer Service for Availability | PLN 1,750.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-657-6
MDL number:
Specific activity:
≥7 units/mg solid
Biological source:
microbial
biological source
microbial
Quality Segment
form
lyophilized powder
specific activity
≥7 units/mg solid
mol wt
~160 kDa
technique(s)
inhibition assay: suitable
color
brown
optimum pH
7.5-8.0
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... xdhA(947116), xdhB(947205), xdhC(945148)
General description
Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Research area: Cell Cycle
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.[1]
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.[1]
Application
Xanthine Oxidase microbial has been used:
Biochem/physiol Actions
Also contains proteolytic activity.
Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism.[3][4] This enzyme is also implicated in caffeine metabolism.[5] XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid.[6] Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.[7]
Physical form
Lyophilized powder containing BSA and sodium glutamate as stabilizers
Preparation Note
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Other Notes
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information Escherichia coli K12 ... xdhA(947116), xdhB(947205), xdhC(945148) | Gene Information - | Gene Information - | Gene Information - |
| specific activity ≥7 units/mg solid | specific activity ≥0.8 units/mg protein | specific activity ≥12 units/mg protein | specific activity ≥0.4 units/mg protein |
| biological source microbial | biological source bovine milk | biological source bacterial (arthrobacter sp.) | biological source - |
| technique(s) inhibition assay: suitable | technique(s) inhibition assay: suitable | technique(s) activity assay: suitable | technique(s) - |
| form lyophilized powder | form ammonium sulfate suspension | form powder | form ammonium sulfate suspension |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Good morning, I purchased xanthine oxidase X2252-25UN for quantifying SOD enzymatic activity. Which buffer should I use to solubilize the enzyme? After solubilization, how should I store the resulting solution?
1 answer-
Helpful?
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Guten Tag, in welchem Lösungsmittel und bis zu welcher Konzentration ist das Enzym gut löslich? Kann Natrium Phosphat Puffer verwendet werden? Danke.
1 answer-
For assay purposes, this product is prepared in 50 mM Potassium Phosphate Buffer, pH 7.5 at 25°C. The material is lyophilized from a solution containing BSA and sodium glutamate. While the solubility has not specifically been tested in sodium phosphate buffer, it is expected to be suitable. Please see the link below to review the assay protocol for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/938/x2252enz.pdfHelpful?
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