V2260

Monoclonal Anti-V5-Peroxidase antibody produced in mouse

clone V5-10, purified immunoglobulin, lyophilized powder

• Synonym(s): Monoclonal Anti-V5
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD03457579
• Conjugate: peroxidase conjugate
• Clone: V5-10, monoclonal
• Application: WB
• Citations: 14

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: V5-10, monoclonal
• form: lyophilized powder
• packaging: vial of 0.5 mL
• concentration: 5-11 mg/mL
• technique(s): western blot: 1:4,000-1:8,000
• storage temp.: 2-8°C

Description

General description

The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.

Application

Suitable for immunoblotting.

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitute with 0.5 mL of distilled water.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Codes: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable