UC9601
UltraClear™ Sequencing Reaction Clean-Up Kit
pkg of 1 plates, sufficient for 1 96-well plate purifications
Synonym(s):
Sequencing Clean-Up Kit, UltraClear Reaction Kit
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About This Item
UNSPSC Code:
41105600
NACRES:
NA.52
Recommended Products
usage
sufficient for 1 96-well plate purifications
packaging
pkg of 1 plates
storage temp.
15-25°C
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General description
The UltraClear Sequencing Reaction Clean-Up Plates offer a rapid and simple method for the clean-up of DNA sequencing reactions in a 96-well format. The plates use ultrafiltration membranes to separate low molecular weight contaminants, such as unincorporated dye terminators, dNTPs, and residual salts from the sequencing reaction products.
Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.
Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.
Features and Benefits
- Effective clean-up of ABI BigDye® chemistries
- Simple centrifugation-based procedure
- Convenient 96-well format
Other Notes
For additional information, please see www.sigma-aldrich.com/sequencing.
Legal Information
BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
UltraClear is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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