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TRUERGD

Sigma-Aldrich

TrueGel3D RGD

Integrin Adhesion Peptide

Synonym(s):

3D cell culture matrix

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

shipped in

dry ice

storage temp.

−20°C

General description

RGD sequences on extracellular matrix proteins (fibronectin, vitronectin) promote cell adhesion to cellular integrin receptors. The thiol group on the N-terminus of TrueGel3D® RGD peptide acts as linker to bind RGD motif to the polymer (Dextran/PVA). The conjugated RGD motif on polymer promotes adhesion of cells with appropriate integrin receptors.
TrueGel3D® is a commercially available polymer-based diverse array of scaffolds for 3D in vitro tumor models. The RGD peptide present in these hydrogels enable cell attachment.

Application

Polymer with cell adhesion motif (RGD) employed to generate cell-adhesive hydrogel

Features and Benefits

Contains cell adhesion motif RGD (Acetyl-Cys-Doa*-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2), which binds to appropriate integrin receptors on cells.

*Doa : 8-amino-3,6-dioxaoctanoic acid

Components

TRUERGD contains RGD degradable polymer lyophilized and water

Legal Information

TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L P Ferreira et al.
Acta biomaterialia, 75, 11-34 (2018-05-29)
Three-dimensional multicellular tumor models are receiving an ever-growing focus as preclinical drug-screening platforms due to their potential to recapitulate major physiological features of human tumors in vitro. In line with this momentum, the technologies for assembly of 3D microtumors are
Steven R Caliari et al.
Nature methods, 13(5), 405-414 (2016-04-29)
There is growing appreciation of the role that the extracellular environment plays in regulating cell behavior. Mechanical, structural, and compositional cues, either alone or in concert, can drastically alter cell function. Biomaterials, and particularly hydrogels, have been developed and implemented

Articles

Co-culture of Tumor and Stromal Cells in TrueGel3D™ Hydrogels

TrueGel3D™ synthetic hydrogel can be used to study co-culture of tumor and stromal cells.

Utilization of TrueGel3D™ Hydrogels to Study Fibroblast Spreading

Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and several other diseases.

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Protocols

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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