T8819
TFIIB human
~5 units/μL, recombinant, expressed in E. coli, solution
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50 UNITS
PLN 2,040.00
PLN 2,040.00
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50 UNITS
PLN 2,040.00
About This Item
PLN 2,040.00
Please contact Customer Service for Availability
recombinant
expressed in E. coli
Quality Level
form
solution
mol wt
~32 kDa
concentration
~5 units/μL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... GTF2B(2959)
Biochem/physiol Actions
General transcription factor for RNA Polymerase II.
The amino terminal region of TFIIB contains a zinc ribbon fold composed of antiparallel Β strands. Mutational studies show that this domain is required for recruitment of RNA Pol II for transcriptional initiation. [1]
Unit Definition
One gel shift unit is the amount of TFIIB required to form 0.2 pmol of TFIIB/TFIID/DNA complex as detected by gel shift.
Physical form
Solution in 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 0.1% Triton® X-100.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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S Hahn et al.
Genes & development, 14(6), 719-730 (2000-03-25)
The function of the conserved zinc-binding domains in the related Pol II- and Pol III-specific factors TFIIB and Brf was investigated. Three-dimensional structure modeling and mutagenesis studies indicated that for both factors, the functional surface of the zinc ribbon fold
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