SRP2001

TFIIA-p55 human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): MGC129969, MGC129970, TF2A1, TFIIA
• NACRES: NA.26
• UNSPSC Code: 12352202
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• Form: frozen liquid
• Mol wt: ~43.6 kDa

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~43.6 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 500 μg/mL
• technique(s): western blot: suitable
• color: clear colorless
• NCBI accession no.: NM_015859
• UniProt accession no.: P52655
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GTF2A1(2957)

Description

Biochem/physiol Actions

The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (a subunit), 19 kDa (b subunit) and 12 kDa (g subunit). The a and b subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (g subunit), retains native TFIIA activity for reconstituted TFIIA, containing all three subunits.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable