SAE0198
Calprotectin, human recombinant
Stabilized heterodimer of fusion tagged S100A9 and S100A8
Synonym(s):
MRP8/14 and S100A8/A9 complex
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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Please contact Customer Service for Availability | 1.750,00 PLN |
About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
≥90% (SDS-PAGE)
1.750,00 PLN
Please contact Customer Service for Availability
General description
This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis Sumo tag is added to the N-terminus of S100A8 and, a 6XHis tag is added to the N-terminus of S100A9. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).
Application
Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease1. This product is a recombinant version of the human protein found endogenously.
Biochem/physiol Actions
Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.1 The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,2 and its antimicrobial activity possibly through chelating of Zn.3 High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.4 A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.5
Physical form
The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.
Preparation Note
Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.
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This Item | |||
|---|---|---|---|
| description Stabilized heterodimer of fusion tagged S100A9 and S100A8 | description - | description The Endostatin protein is a naturally occurring 20 kDa polypeptide derived from the C-terminal portion of type XVIII collagen. | description - |
| assay ≥90% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay >98% (SDS-PAGE and HPLC) | assay - |
| form lyophilized powder | form lyophilized | form - | form liquid |
| shipped in wet ice | shipped in ambient | shipped in dry ice | shipped in wet ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. - | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Signal Word
Warning
Storage Class
11 - Combustible Solids
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
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