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Merck

RAB0100

Human Granulocye-Macrophage Colony Stimulating Factor / CSF2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

GM-CSF

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1 KIT

PLN 2,330.00

PLN 2,330.00


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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... CSF2(1437)
Input:
sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 2.05-500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CSF2(1437)

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This Item
RAB0103RAB0098RAB0569
Gene Information

human ... CSF2(1437)

Gene Information

human ... CSF3(1440)

Gene Information

human ... CSF1(1435)

Gene Information

canine ... CSF2(403923)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

canine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 2.05-500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.69-500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.78-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.13 ng/mL
standard curve range: 0.102-25 ng/mL

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GM-CSF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human GM-CSF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration. GM-CSF plays a crucial role in inflammatory and autoimmune diseases. In neutrophils, it promotes the expression of TLR2 (toll-like receptor) and TLR2-associated interleukin 8 responses, thereby eliminating microbial attack.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificates of Analysis (COA)

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Find documentation for the products that you have recently purchased in the Document Library.

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GM-CSF in inflammation and autoimmunity.
Hamilton JA
Trends in Immunology (2002)
Association of genetic variations in the CSF2 and CSF3 genes with lung function in smoking-induced COPD.
The European Respiratory Journal (2008)
D Margriet Ouwens et al.
Diabetologia, 57(10), 2208-2214 (2014-07-19)
Type 1 diabetes is associated with moderate cognitive decline and cerebral alterations and may lead to an increased risk of dementia, including Alzheimer's disease. This study aimed to investigate the levels of risk markers for Alzheimer's disease in middle-aged patients
Role of toll-like receptor 2 (TLR2) in neutrophil activation: GM-CSF enhances TLR2 expression and TLR2-mediated interleukin 8 responses in neutrophils.
Blood (2002)
Agnieszka Swierczak et al.
Cancer immunology research, 2(8), 765-776 (2014-07-10)
Treatment options are limited for patients with breast cancer presenting with metastatic disease. Targeting of tumor-associated macrophages through the inhibition of colony-stimulating factor-1 receptor (CSF-1R), a key macrophage signaling pathway, has been reported to reduce tumor growth and metastasis, and

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