R7260
Xba I from Xanthomonas badrii
Restriction Enzyme
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
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grade
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
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Specificity
Recognition sequence: 5′-T/CTAGA-3′
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Application
XbaI is a restriction enxyme that cleaves DNA at the recognition site 5′-T/CTAGA-3′ to generate DNA fragments with 5′-cohesive termini.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Xba I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). May cleave at secondary recognition sites under non-optimal conditions.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 0.1 mM PMSF, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.02% polydocanol at 4 °C
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Stephanie L H Miller et al.
The Journal of biological chemistry, 279(32), 33528-33537 (2004-05-25)
The ubiquitin-interacting motif (UIM) is a short peptide motif with the dual function of binding ubiquitin and promoting ubiquitylation. This motif is conserved throughout eukaryotes and is present in numerous proteins involved in a wide variety of cellular processes including
A new specific endonuclease from Xanthomonas badrii..
Zain, B.S., and Roberts, R.J.,
Journal of Molecular Biology, 115, 244-255 (1977)
Miyuki Watabe et al.
The Ulster medical journal, 77(3), 168-174 (2008-10-30)
In Northern Ireland over the last 7 years, there is a mean of 41.9 laboratory reports per annum of human gastrointestinal infection (range 19-54) caused by Escherichia coli O157:H7. In the preceding years 1992-1996, reports were 5.4 per annum, whereas
E Malyguine et al.
Gene, 8(2), 163-177 (1980-01-01)
The specificity of XbaI, SalI, HhaI, PstI, BamHI and SstI is relaxed in the presence of an organic solvent, such as 20% glycerol or 8% dimethylsulfoxide (DMSO). This alteration, very pronounced in some cases, requires an excess of enzyme, varies
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
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