Skip to Content
Merck

Key Documents

View All Documentation

Skip To

P9743

pT7-FLAG™-4 Expression Vector

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
10 μg
Please contact Customer Service for Availability
1.690,00 PLN

About This Item

NACRES:
NA.32
UNSPSC Code:
41106610

1.690,00 PLN

Please contact Customer Service for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Segment

technique(s)

molecular cloning: suitable

shipped in

dry ice

storage temp.

−20°C

Application

pT7-FLAGa-4 is a 6451 bp Escherichia coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAGÒ fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORF-FLAG fusion constructs.

Legal Information

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
P1243E5280P3871
pT7-FLAG™-4 Expression Vector

Sigma-Aldrich

P9743

pT7-FLAG™-4 Expression Vector

pT7-FLAG™-2 Expression Vector

Sigma-Aldrich

P1243

pT7-FLAG™-2 Expression Vector

pT7-FLAG™-MAT-Tag®-1 Expression Vector

Sigma-Aldrich

E5280

pT7-FLAG™-MAT-Tag®-1 Expression Vector

pT7-FLAG-SBP™-1 Expression Vector

Sigma-Aldrich

P3871

pT7-FLAG-SBP™-1 Expression Vector

description

-

description

-

description

-

description

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

  1. pT7-FLAG-4のVectorに遺伝子を導入して導入遺伝子-FLAGごと別のVectorに移動することを考えているのですが、FLAGよりもC-Terminal側の制限酵素サイトの情報はありますでしょうか。ご教示いただけると幸いです。

    1 answer

    1. Only restriction enzyme information at the multiple cloning site (MCS) is available, as shown in the technical bulletin. There isn't a restriction enzyme site available at the C-terminal end of the FLAG tag before the stop codon.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/048/p9743bul.pdf

      Helpful?

Reviews

No rating value

Active Filters