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Phosphoglucose Isomerase from Bacillus stearothermophilus

Name: Phosphoglucose Isomerase from <I>Bacillus stearothermophilus</I>
Brand: SIGMA
Price: 1793.5 PLN

lyophilized powder, 300-1,000 units/mg protein

Synonym(s):

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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Pack Size	SKU	Availability	Price
100 units		Please contact Customer Service for Availability	PLN 2,110.00 PLN 1,793.50
250 units		Please contact Customer Service for Availability	PLN 4,010.00 PLN 3,408.50

About This Item

CAS Number:

9001-41-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-603-1

MDL number:

MFCD00131887

EC Number:

5.3.1.9 (BRENDA, IUBMB)

Specific activity:

300-1,000 units/mg protein

PLN 1,793.50

List PricePLN 2,110.00Save 15%

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

300-1,000 units/mg protein

mol wt

189 kDa

composition

Protein, ≥60% biuret

storage temp.

−20°C

Description

General description

The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.

Application

Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus.

The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.[1]

Biochem/physiol Actions

Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Physical form

lyophilized powder containing Tris buffer

Other Notes

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C.

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This Item	P9544	P5381	P2783
Sigma-Aldrich P5538 Phosphoglucose Isomerase from Bacillus stearothermophilus	Sigma-Aldrich P9544 Phosphoglucose Isomerase from rabbit muscle	Sigma-Aldrich P5381 Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich P2783 Phosphotransacetylase from Bacillus stearothermophilus
specific activity 300-1,000 units/mg protein	specific activity ≥200 units/mg protein	specific activity ≥400 units/mg protein (biuret)	specific activity ≥3,000 units/mg protein (biuret)
form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder
mol wt 189 kDa	mol wt -	mol wt tetramer 119,500 Da±600	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
composition Protein, ≥60% biuret	composition protein, 70-100% biuret	composition -	composition Protein, ≥20%