P4399

β-Lactamase from Enterobacter cloacae

Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)

• Synonym(s): β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
• CAS Number: 9073-60-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• eCl@ss: 32160702
• Specific activity: 6-18 units/mg protein (using benzylpenicillin)

Properties

• type: Type III
• form: lyophilized powder
• specific activity: 6-18 units/mg protein (using benzylpenicillin)
• composition: Protein, ~10%
• storage temp.: 2-8°C

Description

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4399 is produced from Enterobacter cloacae.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Physical form

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Faceshields, Gloves