M8440

Anti-MCH antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): MCH Antibody - Anti-MCH antibody produced in rabbit, Mch Antibody, Anti-Melanin-Concentrating Hormone
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD03791841
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, ELISA (i)
• Citations: 19

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): indirect ELISA: 1:2,500, microarray: suitable
• UniProt accession no.: P20382
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PMCH(5367)

Description

General description

Melanin-concentrating hormone (MCH) is a circulating 19-amino acid residue cyclic neuropeptide present in hypothalamus. In mammals, MCH is a, encoded by the precursor pro-melanin concentrating hormone (PMCH). It was first discovered in fish and subsequently in mammalian (rat). MCH perikarya is confined largely to the lateral hypothalamus area (LHA) and the adjacent zon incerta with extensive neuronal projections throughout the brain. MCH is detected in basal ganglia, neocortex, cerebellum, thymus, brown adipose tissue, duodenum, and testis. The structure of MCH is highly conserved among species.

Immunogen

synthetic peptide corresponding to human melanin-concentrating hormone conjugated to KLH. The MCH sequence is identical in many species including rat, mouse, and pig.

Application

Anti-MCH antibody may be used at a working dilution of 1:30000 for RIA. For immunohistochemistry of mouse hypothalamus sections, a working antibody dilution of 1:200 may be used. The antibody has been used for immunoblotting in cytoplasmic extracts of T cells at a concentration of 5.7 μg/ml. The antibody is suitable for protein microarray.

Anti-MCH antibody produced in rabbit has been used in radioimmunoassay and immunohistochemistry.

Biochem/physiol Actions

MCH mRNA is overexpressed and up-regulated during fasting in the hypothalamus of ob/ob mice as well as rats. Injection of MCH into the lateral ventricles in rats promote increased food consumption. In addition, knockout mice lacking MCH gene are lean and hypophagic and show an increase in metabolic rate. In contrast, overexpression of MCH in transgenic mice leads to obesity and insulin resistance.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: nwg